SUPPORT

• Infographics on Women in Drug Delivery (PDF – Gender in DD)
• Drug Delivery Guess Who? (PDF – Guess Who Drug Delivery)
• Ins and Outs of for tenure-tracks faculty positions (.pdf)
• Various Color Pallets that can be seen better by the color blind (.pdf)
• Select Lecture Slides
• Protocols

SELECT LECTURE SLIDES

• • • Timeline updated Sept 2023 – Stiepel-et-al.-timelines-July 2024(PDF) – Stiepel-et-al.-timelines- July 2024 (Google Slides)
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  • Controlled release defined and illustrated with release of drug from polymeric particles from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421  
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  • Graphic of local versus systemic administration of drug from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421  
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  • PEGylation and its benefits from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421  
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  • Active versus passive targeting of drug carriers from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421
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  • Enhanced permeability and retention (EPR) explained from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421
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  • Immunological response to PEG from Stiepel et al. 2022 https://doi.org/10.1002/btm2.10421
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  • Explaining HLB used for emulsions
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  • Passive and Active Transport: Methods which drugs can be taken up by cells in the body
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  • Explaining carrier saturation and transporter kinetics
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  • The in’s and out’s of applying for tenure-track faculty positions (.pdf)

PROTOCOLS

Particle Fabrication and Characterization

• • Single emulsion sonicated nanoparticles (.docx .pdf)
  • Single emulsion homogenized nanoparticle (.docx .pdf)
  • Double emulsion nanoparticles using a homogenizer (.docx .pdf)
  • Double emulsion sonicated nanoparticles (.docx .pdf)
  • Fluorescamine Assay to Determine Protein Content in Polymer nanoparticles (.docx .pdf)
  • Measurement of endotoxin (pdf)

Cell Culture

• • Caco-2 Cell Culture Protocol (.docx .pdf)
  • RAW Macrophage Cell Maintenance (.docx .pdf)
  • Freezing down mammalian cells (.docx .pdf)
  • Working Volumes for Tissue-Culture Vessels (.xls .pdf)
  • MTT viability assay (.docx .pdf)
  • Greiss Nitric Oxide (NO) assay (.docx .pdf)
  • Standard curve frequently asked questions (.docx .pdf)
  • BCA Kit Quantification of Proteins (.docx .pdf)
  • Subculture and cryopreservation of Leishmania donovani (.docx .pdf)

Florescent Staining

• • Procedure for live dead cell analysis with cell tracker and PE (.docx .pdf)
  • Procedure for apoptosis/necrosis of non-fixed adherent cells (.docx .pdf)
  • Adherent cell ROS staining for flow cytometry (.docx .pdf)
  • Adherent Superoxide Staining for flow cytometry (.docx .pdf)

Monocytes

• • • Isolation of human monocytes (.docx .pdf)

Immunoassays

• • Spleen, Lymph Node, and BAL Harvest Protocol for ELISpot, Cytokine ELISA, and Flow (.pdf)
  • OVA ELISA for Serum Antibodies (.pdf)
  • Murine Bone Marrow-Derived Macrophage (BMM) culture (.pdf)
  • Multi-color cell surface staining (.pdf)
  • T cell CFSE Staining (.pdf)